Table 1 Summary of antisera and immunohistochemical findings in conventional and MELF pattern tumor components

From: Expression of cell cycle regulatory proteins in endometrial adenocarcinoma: variations in conventional tumor areas and in microcystic, elongated and fragmented glands

Antibody

Source; dilution

Conventional tumor areas

MELF tumor areas

Cyclin D1

Dako, NSW, Australia; 1:100

Variable expression in 20 tumors (>50% cells positive in eight cases). Peripheral/basal glands more consistently stained.

Strongly positive in 20 cases, sometimes in contrast to unstained adjacent or contiguous conventional tumor areas.

β-Catenin

Novocastra, Newcastle, UK; 1:4000

Membrane labeling in 18 tumors, often with cytoplasmic staining in peripheral/basal glands. Focal nuclear staining in six cases. Squamous morules positive.

Complete loss of membrane staining in most areas, usually with weak cytoplasmic reactivity. Focal nuclear staining in two cases.

p16

Santa Cruz, CA, USA; 1:50

All cases at least focally stained (>50% cells positive in 11 cases). Squamous morules positive. Variable distribution but central glands more uniformly stained.

Moderate-to-strong immunoreactivity in 20 cases, sometimes in contrast to unstained adjacent or contiguous conventional tumor areas.

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