Figure 1
From: Application of COLD-PCR for improved detection of KRAS mutations in clinical samples
Design of the full co-amplification at lower denaturation temperature PCR COLD-PCR assay for KRAS mutations detection. (a) Nucleotide sequence of the 98-bp PCR product and melting temperature (Tm) profiles of the wild-type and mutant sequences at each of the six nucleotides of codons 12–13. Arrows indicate primer sequences (underlined). Sequence of codons 12–13 is indicated in red. Figure legends indicate Tm profiles for wild-type (‘wild’) and mutant (‘m’ and nucleotide number, eg ‘m25’) sequences. (b) Description of the PCR reaction conditions used in this study for conventional PCR and COLD-PCR protocols.
