Figure 2
From: Application of COLD-PCR for improved detection of KRAS mutations in clinical samples
Sensitivity determined by dilution study. (a) Representative pyrograms of serial-dilutions study with conventional PCR and co-amplification-at-lower denaturation-temperature PCR (COLD-PCR). (b) Comparison of pyrosequencing results from undiluted to1:32 dilution after conventional PCR and COLD-PCR (bars), and enhancement of COLD-PCR in serial dilutions (line).
