Table 2 Comparison of mutant-to-wild-type-ratio by conventional PCR and COLD-PCR in fresh bone-marrow aspirate samples
From: Application of COLD-PCR for improved detection of KRAS mutations in clinical samples
No. | Age | Diagnosis (FAB classification) | Mutation type | Nucleotide change | Tumor cell count (%) | Ratio by conventional PCR (%)a | Ratio by COLD-PCR (%) | Enhancement by COLD- PCR (fold) |
|---|---|---|---|---|---|---|---|---|
1 | 57 | MDS | G12D | GGT to GAT | 4 | 1.7 | 1.8 | 1.0 |
2 | 84 | AML (M2) | G12D | GGT to GAT | 31 | 23.7 | 55.3 | 2.3 |
3 | 48 | MDS | G13S | GGC to AGC | 2 | 6.2 | 12.1 | 1.9 |
4 | 33 | AML | G12V | GGT to GTT | 83 | 28.9 | 51.6 | 1.8 |
5 | 63 | MDS | G12S | GGT to AGT | 4 | 57.7 | 67.7 | 1.2 |
6 | 60 | AML (M4) | G12D | GGT to GAT | 52 | 2.4 | 3.1 | 1.3 |
7 | 50 | AML (M2) | G12D | GGT to GAT | 40 | 47.2 | 66.0 | 1.4 |
8 | 57 | CMML | G12D | GGT to GAT | 10 | 64.1 | 68.0 | 1.1 |
9 | 35 | AML (M5) | G12V | GGT to GTT | 75 | 39.4 | 57.0 | 1.5 |
10 | 51 | AML | G12D | GGT to GAT | 36 | 20.7 | 31.2 | 1.5 |
11 | 31 | AML (M5) | G12D | GGT to GAT | 80 | 19.7 | 23.3 | 1.2 |
12 | 78 | CMML | G12R | GGT to CGT | 14 | 50.3 | 54.5 | 1.1 |
13 | 78 | AML | G12D | GGT to GAT | 90 | 44.7 | 40.6 | 0.9 |
14 | 72 | MDS | G12R | GGT to CGT | 8 | 16.5 | 21.9 | 1.3 |
15 | 77 | MDS | G12R | GGT to CGT | 80 | 32.5 | 35.0 | 1.1 |
16 | 79 | AML (M4) | G12D | GGT to GAT | 35 | 24.7 | 38.8 | 1.6 |
48 | 49 | AML (M2) | G12D | GGT to GAT | 3 | 0.0 | 8.1 |
