Table 3 Comparison of mutant-to-wild-type-ratio by conventional PCR and COLD-PCR in formalin-fixed paraffin-embedded tissue samples

No.	Age	Sample type	Diagnosis	Mutation type	Nucleotide change	Tumor percentage ^a	Ratio by conventional PCR (%)	Ratio by COLD-PCR (%)	Enhancement by COLD- PCR (fold)
17	66	Bladder	Colon cancer metastasis	G12D	GGT to GAT	60	38.0	45.2	1.2
18	45	Liver	Colon cancer metastasis	G12V	GGT to GTT	30	20.2	40.0	2.0
19	54	Colon	Colon cancer metastasis	G12A	GGT to GCT	10	1.4	6.4	4.7
20	67	Lymph node	Rectal cancer metastasis	G12V	GGT to GTT	70	42.0	56.0	1.3
21	75	Colon and rectum	Rectal cancer metastasis	G12D	GGT to GAT	80	43.0	46.5	1.1
22	45	Liver	Colon cancer metastasis	G12C	GGT to TGT	80	53.2	53.7	1.0
23	77	Colon	Colon cancer metastasis	G12V	GGT to GTT	90	52.9	17.2	0.3
24	54	Liver	Colon cancer metastasis	G12S	GGT to AGT	80	48.0	46.3	1.0
25	46	Lung	Colon cancer metastasis	G12D	GGT to GAT	80	58.6	64.7	1.1
26	66	Liver	Rectal cancer metastasis	G12D	GGT to GAT	50	35.8	76.2	2.1
27	72	Colon	Adenocarcinoma	G12D	GGT to GAT	90	69.6	47.8	0.7
28	37	Colon	Adenocarcinoma	G12V	GGT to GTT	50	42.6	90.1	2.1
29	66	Rectum	Adenocarcinoma of rectum	G12D	GGT to GAT	5	17.2	45.7	2.7
30	76	Colon	Adenocarcinoma	G13D	GGC to GAC	50	43.3	56.1	1.3
31	76	Rectum	Adenocarcinoma	G12R	GGT to CGT	50	17.5	37.0	2.1
32	69	Colon	Adenocarcinoma	G12V	GGT to GTT	40	19.2	49.0	2.6
33	76	Colon	Adenocarcinoma	G12V	GGT to GTT	80	30.8	51.7	1.7
34	55	Colon	Adenocarcinoma	G12V	GGT to GTT	60	42.5	60.0	1.4
35	45	Colon	Adenocarcinoma	G12D	GGT to GAT	70	52.2	61.0	1.2
36	78	Colon	Adenocarcinoma	G12D	GGT to GAT	80	48.4	88.6	1.8

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