Figure 2
Western blotting analysis for p-FRS2αTyr196 and p-ERK1/2Thr202/Tyr204 on eight selected chordomas (CHD). The six chordomas on the left-hand panel were positive by immunohistochemistry for at least one of the FGFRs and showed a band corresponding to p-FRS2α and p-ERK1/2 by western blotting. The middle panel of two chordomas, found to be negative for any FGFR by immunohistochemistry, did not display a band for p-FRS2α. Nonetheless, a band was detected by western blotting for p-ERK1/2 in these two cases. In the right-hand panel, SKOV3 cell line, stimulated with basic FGF, was used as a positive control. The membranes were stripped and re-probed with total ERK1/2 and an anti-GAPDH antibody to ensure an even loading of proteins in each lane.
