Table 1 Details of antibodies used for immunohistochemistry

From: Analysis of the fibroblastic growth factor receptor-RAS/RAF/MEK/ERK-ETS2/brachyury signalling pathway in chordomas

Antibody/clone

Source

Antigen retrieval

Dilution

Incubation time and temperature

Method

Cytokeratin 19 clone b170

Novocastra, Peterborough, UK

Protease 20 min

1:100

30 min at 37°C

Ventana NexES autostainer

Brachyury (H-210): sc-20109

Santa Cruz, CA, USA

Pressure cooker 2 min

1:50

30 min at 37°C

Ventana

FGFR1/Flg (C-15): sc-121

Santa Cruz

Protease 14 min

1:150

30 min at 37°C

Ventana

FGFR2/Bek (C-17): sc-122

Santa Cruz

Protease 14 min

1:100

30 min at 37°C

Ventana

FGFR-3 (C-15): sc-123

Santa Cruz

Protease 14 min

1:100

Overnight at 4°C in a humid chamber

Manual staining

FGFR-4 (C-16): sc-124

Santa Cruz

Protease 14 min

1:50

30 min at 37°C

Ventana

Back to article page