Figure 5
CD90 in urine. Single-ion traces of the mass from heavy (d9, pre-op urine) and light (d0, post-op urine) ICAT-labeled peptides indicate that PSA and CD90 are detectable in prostate cancer patient urine. The elution profiles (single-ion traces of the peptide mass over time) of heavy-labeled peptide masses and those of the corresponding light-labeled peptide masses are highlighted. The detected peptide sequences are shown below the traces. The weight difference of 18 in PSA is due to the two-labeled Cys residues. Both PSA (more abundant than CD90, y-axis) and CD90 show a similar trend wherein both proteins are present in the pre-op sample but below detection limit in the post-op sample.
