Figure 2


Immunohistochemical analysis of minichromosome maintenance protein 7 (MCM7) expression in tissue samples. (a) As positive and negative controls, mouse testicular specimens were stained with anti-MCM7 antibodies. MCM7 is specifically expressed in the nuclei of spermatogonia and spermatocytes. As the cells in the germinal layer differentiated, less staining of MCM7 was observed in spermatids, and fully differentiated sperms completely lacked MCM7 expression. (b) Representative nuclear staining patterns of MCM7 in tumor cells. MCM7 staining is observed by brown chromogen staining. All micrographs were taken at original magnification of × 10 objective.