Table 1 Antigen and antibody clone, source, dilutions, and antigen retrieval conditions
From: IgG4 plasma cells in inflammatory myofibroblastic tumor: inflammatory marker or pathogenic link?
Antibody | Clone | Company | Catalogue no. | Dilution | Pre-treatment |
|---|---|---|---|---|---|
ALK-1 | ALK-1 | Dako Cytomation; Carpinteria, CA | N1614 | Prediluted | HIER Dako EDTA (pH 9.0) |
IgG | Rabbit polyclonal | Dako Cytomation; Carpinteria, CA | A0423 | 1:20 000 | HIER Dako citrate (pH 6.0) |
IgG4 | HP 6025 | Invitrogen; Camarillo, CA | 05-3800 | 1:200a | HIER Zymed EDTA (pH 8.0) |
SMA | 1A4 | Dako Cytomation; Carpinteria, CA | M0851 | 1:100 | HIER Zymed EDTA (pH 8.0) |
