Figure 1


Photographs depicting prostate cancer tissue immunohistochemically stained for MSMB and CRISP3 protein expression (left), with corresponding markup images generated by the IHC-MARK algorithm (right), respectively. The algorithm recognizes cancer cells by their morphological properties, and marks it with a corresponding color. The nuclei of IHC-MARK negative tumor cells are seen in blue; nuclei of IHC-MARK-positive tumor cells in red; and cytoplasm of tumor cells in orange. Tumor areas are defined by green lines. MSMB and CRISP3 expressions were subsequently stratified into high and low subgroups using classification regression tree analyses (Figures 3 and 5a). Photographs were generated at an original magnification of × 20.