Figure 2

Comparison of MYC copy number in primary breast cancer, lymph node metastases upon initial diagnosis, and subsequent metastases on autopsy by two-color fluorescence in situ hybridization (MYC in red and centromere 8 in green) for cases 11 (a–d) and 12 (e–h). At the time of diagnosis, both the primary (a, case 11; e, case 12) and axillary lymph node metastases (b, case 11; f, case 12) were characterized by a similar MYC duplication. However, systemic metastases harvested at autopsy for both cases (c and d, case 11; g and h, case 12) acquired an MYC amplification. Metastases for case 11 include axillary lymph node (c) and lung (d), while case 12 include two separate brain metastases (g and h). See Table 3 for fluorescence in situ MYC to centromere 8 ratios for each primary and corresponding metastases.