Figure 5
Western blot analysis of prenylated proteins including HDJ2 and KRAS as well its downstream signals. Increased non-farnesylated HDJ2 protein (upper black arrow) and decreased membrane-bound KRAS protein were observed in the siRNA AKR1B10 pancreatic cancer cell line when compared with the scrambled siRNA control. Increased levels of membrane-bound E-cadherin (lower black arrow) and decreased levels of KRAS downstream effectors, phosphor-ERK and phosphor-MEK1/2 were also found. Flottin-2 was used as a loading control for membrane-bound proteins, whereas β-actin is used for cytosolic proteins.
