Table 1 Primary antibodies and conditions used for immunohistological studies

From: The spectrum of B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell lymphoma and classical Hodgkin lymphoma: a description of 10 cases

Marker

Clone

Dilution

Antigen retrieval

Source

Interpretation (positive)

CD45RB

PD7/26+2B11

1:500

MW; CB

Dako

>50%

CD20

L26

1:1200

MW; CB

Dako

>50%

CD3

SP7

1:200

S; CB

Lab Vision

>50%

CD30

BerH2

1:900

S; CB

Dako

>50%

CD15

Leu M1

1:50

MW; CB

BD

Any

LMP-1

CS1-4

1:200

S; CB

Dako

>10%

CD79a

124

1:400

MW; CB

Dako

>50%

BCL-6

PG-B6P

1:100

T+S; TRIS

Dako

>50%

P63

4A4

1:250

PC; CB

Lab Vision

>50%

BOB-1

BOB1

1:80

PC; CB

Novocastra

>30%

Ki-67

MIB-1

1:4800

PC; CB

Dako

%

BCL-2

124

1:400

MW; CB

Dako

>30%

IRF4/MUM-1

MUM1P

1:1200

S; CB

Dako

>30%

PAX5

DAK-PAX5

1:400

S; CB

Dako

>50%

OCT2

OCT-2

1:500

MW; EDTA

Sta. Cruz

>30%

CD23

SP23

1:400

MW; CB

Lab Vision

>25%

  1. DAKO: Carpinteria, CA, USA; Lab Vision Corporation: Fremont, CA, USA; Novocastra: Newcastle Upon Tyne, UK; Becton-Dickinson (BD) Biosciences: Mountain View, CA, USA; Sta. Cruz: Santa Cruz, CA, USA; MW: microwave oven; PC: pressure cooker; S: steamer; T: trypsin; CB: citrate buffer.