Table 2 Summary of KRAS and TP53 mutation analysis, microsatellite analysis and immunohistochemistry

Case	Sex/age	Mutation analysis		Immunohistochemistry				MSI	LOH
		TP53	KRAS	TP53	CDKN1A	SMAD4	CTNNB1
PJS1	M/45	WT	WT	−	0	+	Membranous	MSS	D17S250, D5S346
T1	M/48	S & MS	WT	+	2	+	Membranous	MSS
T2	F/68	WT	WT	−	0	+	Nuclear	MSS
T3	M/61	WT	WT	−	2	−	Membranous	MSS	D17S250
T4	F/19	WT	WT	−	0	+	Membranous	MSS
T5	M/33	NA^a	NA^a	−	1	+	Membranous	MSS

Abbreviations: M, male; F, female; PJS, Peutz-Jeghers syndrome; WT, wild type; S, silent mutation; MS, missense mutation; MSI, microsatellite instability; MSS, microsatellite stable; LOH, loss of heterozygosity; NA, not applicable.
For TP53-normal negative labeling; +, positive labeling. For CDKN1A: 0, absent labeling; 1, weak positive labeling; 2, strong positive labeling. For SMAD4-absent labeling; +, normal positive labeling. For CTNNB1: N, normal membraneous (negative); F, focal nuclear labeling (positive).
^aInsufficiënt DNA.

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