Table 2 Results of DNA and immunohistochemical analyses

From: Immunohistochemistry with a mutation-specific monoclonal antibody as a screening tool for the BRAFV600E mutational status in primary cutaneous malignant melanoma

Sample

BRAFV600E status

Immunohistochemical analyses

M1

M2a

(GTG to AAG)

M3

M4

M5

M6

M7

M8a

(GTG to ATG)

M9

+

+

M10

+

+

M11

+

+

M12

M13

M14

M15

M16

M17

M18

M19

M20b

+

+

M21

M22

+

+

M23

M24

M25

M26

+

M27

M28

M29

M30

M31

M32

M33

+

+

M34

M35

C1

+

+

C2

+

+

C3

+

+

C4

+

+

C5

+

+

C6

+

+

C7

+

+

C8

+

+

C9

+

+

C10

+

+

  1. For BRAFV600E mutation status, cases were marked as positive/negative using a combination of direct DNA Sanger sequencing and allele-specific PCR.
  2. aCases with a mutation in BRAF other than V600E.
  3. bCase presented with both nuclear and cytoplasmic staining.