Figure 4 | Modern Pathology

Figure 4

From: Targeted next generation sequencing of clinically significant gene mutations and translocations in leukemia

Figure 4

Coverage of clinically important genes and translocation areas. Blue: NB4, red: MV4–11, green: OCI-AML3, purple: K562, and orange: kasumi-1. (a) FLT3 coverage for all cases in the region of the FLT3 ITD. Average coverage was 116-fold. (b) NPM1 coverage in the area surrounding exon 11 that generally harbors insertions. Average coverage was 174-fold. (c) Coverage of 1 kb in ABL1 gene flanking the translocation site (middle) in K562 cells. Note that coverage in K562 increases dramatically at the breakpoint, likely indicating a copy number change in this region. (d) In contrast, the 1-kb area surrounding the t(15;17) RARA breakpoint in NB4 cells show no evidence of copy number change.

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