Table 2 Next generation sequencing findings in commonly tested genes

From: Targeted next generation sequencing of clinically significant gene mutations and translocations in leukemia

 

Reported translocation

Captured gene location

Partner gene location

FLT3 ITD

FLT3 D835

NPM1

KIT

OCI-AML3

N/A

  

−

−

+

−

NB-4

t(15;17) PML-RARA

Chr17: 38502182

Chr15: 74326368

−

−

−

−

Kasumi-1

t(8:21) AML-ETO

Undetermineda

Undetermineda

−

−

−

+

K562

t(9;22) BCR-ABL

Chr9: 133607147

Chr22: 23632742

−

−

−

−

MV4-11

t(4;11) MLL-AF4

Chr11: 118353675

Chr4: 88009085

+

−

−

−

Case 100

t(9;11) MLL-AF9

Chr11: 118354598

Chr9: 20355515

−

−

−

−

  1. aThe translocation breakpoint in this case occurred outside of the RUNX1 capture region (truncated because of design limitations) and was not sequenced.
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