Table 1 Antibodies used in this study
Marker | Source | Type | Dilution | Antigen retrieval |
|---|---|---|---|---|
Calretinin | Zymed (South San Francisco, CA, USA) | PAb (rabbit) | 1:20 | Yes (citrate) |
Pan-keratin | Dako (Carpinteria, CA, USA) | AE1/AE3 MAbs | 1:50 | Yes (citrate) |
Keratin 7 | Dako | OV-TL 12/30 | 1:100 | Yes (enzymatic digestion) |
Keratin 5/6 | Dako | D5/16B4 MAb | 1:100 | Yes (citrate) |
WT1 | Dako | 6F-H2 MAb | 1:40 | Yes (Tris-EDTA) |
Podoplanin | Signet Laboratories (Dedham, MA, USA) | D2-40 MAb | 1:25 | Yes (Tris-EDTA) |
Mesothelin | Novocastra (Newcastle-upon-Tyne, UK) | 5B2 | 1:30 | Yes (Tris-EDTA) |
MOC-31 | Dako | MAb | 1:50 | Yes (citrate) |
CEA | Lab Vision (Fremont, CA, USA) | PAb (rabbit) | 1:200 | No |
CD15 | Becton-Dickinson (Mountainview, CA, USA) | Leu-M1 MAb | 1:40 | Yes (Tris-EDTA) |
TAG-72 | BioGenex (San Ramon, CA, USA) | B72.3 MAb | 1:300 | No |
TTF-1 | Dako | 8G7G3/1 MAb | 1:25 | Yes (citrate) |
