Figure 1
From: Correlation of DLC1 gene methylation with oncogenic PIK3CA mutations in extramammary Paget's disease
Methylation status of the CpG island region in the deleted in liver cancer 1 (DLC1) promoter of extramammary Paget's disease patients. (a) Schematic depiction of the DLC1 promoter-associated CpG island, which spans the region from −278 to +16, with respect to the ATG start site (+1). Regions analyzed by MS-HRM and bisulfite genomic sequencing (Bis-DLC1) are shown. The Bis-DLC1 region encompassed 292 bp and contained 35 CpG dinucleotides. (b) Examples of a highly methylated DLC1 CpG island (sample S3, left) and an unmethylated CpG island (sample N1, right), as determined by bisulfite sequencing analysis. Arrows indicate the positions of CpG dinucleotides. (c) Methylation patterns of the Bis-DLC1 region of the DLC1 CpG island in a normal tissue sample, and six extramammary Paget's disease tumors and hyperplasia samples that were identified by MS-HRM as methylated. Methylated and unmethylated CpG sites are shown as solid circles and open circles, respectively.
