Table 2 Primers designed to detect E6 mRNA expression of the low-risk HPV types
HPV type | Sequence | Target gene | Amplicon size (bp) |
|---|---|---|---|
HPV6 | F: 5′-GTGCAAGACGTTTAATCTATCTATGC-3′ R: 5′-TATGAATAAATCTCTGCTGTGGTC-3′ | E6 | 91 |
HPV11 | F: 5′-CACACTCTGCAAATTCAGTGCG-3′ R: 5′-CACACAACCTTTAGGTTCTTATAGGC-3′ | E6 | 92 |
snRNP U1A | F: 5′-CAGTATGCCAAGACCGACTCAGA-3′ R: 5′-GGCCCGGCATGTGGTGCATAA-3′ | Housekeeping gene | 215 |
