Table 1 Protocol used for each single antibody
Antibody | Company | Clone | Source | Dilution | Immunostainer | Antigen retrieval | Incubation | Detection |
|---|---|---|---|---|---|---|---|---|
Confirm ER Rmab | VMS | SP1 | Rmab | PD | BenchMarch XT VMS | CC1 pH 8.2 × 30′ | 37 °C × 20 min | UltraView Universal Dab Detection |
Confirm PR Rmab | VMS | 1E2 | Rmab | PD | BenchMarch XT VMS | CC1 pH 8.2 × 30′ | 37 °C × 16 min | UltraView Universal Dab Detection |
Confirm anti-Ki-67 | VMS | K-2 | Mmab | PD | BenchMarch XT VMS | CC1 pH 8.2 × 30′ | 37 °C × 16 min | UltraView Universal Dab Detection |
Pathway HER-2/neu | VMS | 4B5 | Rmab | PD | BenchMarch XT VMS | CC1 pH 8.2 × 30′ | 37 °C × 16 min | UltraView Universal Dab Detection |
Confirm anti-p53 | VMS | D07 | Mmab | PD | BenchMarch XT VMS | CC1 pH8,2 × 30′ | 37 °C × 40 min | UltraView Universal Dab Detection |
P16 | SCBT | JC8 | Mmab | 1:100 | BenchMarch XT VMS | CC1 pH 8.2 × 30′ | 37 °C × 20 min | UltraView Universal Dab Detection |
Cytokeratin 5 | Leica | XM26 | Mmab | 1:100 | Bond MaX Leica | ER2 pH 6.0 × 30′ | RT × 15 min | Bond Polymer Refine Detection |
RB gene protein | Leica | 13A10 | Mmab | 1:50 | Bond MaX Leica | ER2 pH 9.0 × 30′ | RT × 15 min | Bond Polymer Refine Detection |
