Figure 2

Analysis of invasion and metastasis of ALDH1A1^high cells and the expression of invasion-associated genes by ALDH1A1^high cells. (a) In vitro invasion images of Transwell assay show increased number of ALDH1A1^high cells. (b) Quantitative analysis shows significant difference between invasion by ALDH1A1^high and that by ALDH1A1^low cells. (c) Enhanced lung metastasis of ALDH1A1^high cells injected into nude mice. (d) Quantitative real-time polymerase chain reaction (qRT-PCR) shows high levels of vimentin, MMP2, MMP7 and MMP9 expressions and low level of E-cadherin (CDH1) expression in ALDH1A1^high compared with those in ALDH1A1^low cells. ALDH1A1, aldehyde dehydrogenase 1A1; MMP, matrix metalloproteinase.