Figure 5
Distribution of the viral and cellular markers E4, L1 and minichromosome maintenance protein 7 (MCM7) in the adjacent epithelium of a squamous cell carcinoma from a kidney transplant recipient (patient 17, ear). The top pictures shows the scan of the tissue sections (hematoxylin and eosin (H&E) staining) of the squamous cell carcinoma (left picture; scale bar: 500 μm) and its edge (right picture; scale bar: 1000 μm). In the lower panel, the same sections were stained using antibodies to E4 (green) (middle picture) and serial sections were stained with antibodies to L1 (red) (right picture). The region shown corresponds to the red square highlighted in the H&E image. Scale bars: 50 μm. All sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue) to visualize cell nuclei. The inset in the lower left picture shows the cytopathic effects typical of a productive β-human papillomavirus (HPV) infection (scale bar: 20 μm).
