Table 1 Specifications of primary antibodies, source, epitope retrieval, and criteria for the cutoff

From: Follicular lymphoma in young adults: a clinicopathological and molecular study of 200 patients

Antigen

Clone

Dilution

Source

Epitope retrieval

Cutoff

CD23

SP23

1:200

Neomarkers

M.POL

Any

CD20

L26

1:1200

DAKO

MM.ABC

Any

CD10

56C6

1:200

Novocastra

S.POL

Any

CD3

565

1:800

Novocastra

MM.ABC

Any

BCL2

124

1:2000

DAKO

MM.ABC

Any

BCL6

564

1:2400

Novocastra

S.POL

Any

Ki-67

MIB-1

1:4800

DAKO

S.POL

≥50%

kappa

A8B5

1:200

DAKO

MM.ABC

Any

lambda

N10/2

1:400

Neomarkers

S.POL

Any

MDM2

IF2

1:2400

Zymed

S.POL

≥20%

MUM1/IRF4

MUM1P

1:1200

DAKO

S.POL

Any

  1. Abbreviations: FL, MM.ABC: microwave, mouse avidin biotin complex; M.POL: microwave, polymer; S.POL: steamer, polymer.
  2. DAKO (Carpinteria, CA, USA); Neomarkers/Lab Vision (Fremont, CA, USA); Novocastra (Newcastle, UK); Zymed (San Francisco, CA, USA).
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