Table 1 Sources of antibodies and conditions of immunohistochemical staining
Antibody | Source | Clone | Dilution | Pretreatment cook buffer and conditions | Incubation time and temperature |
|---|---|---|---|---|---|
Pancytokeratin | Beckmann-Coulter | KL-1 | 1:100 | Cook buffer CC1, 36 min at 95 °C | RT, 80 min |
EMA | DAKO | E29 | 1:200 | Cook buffer CC1, 36 min at 95 °C | 37 °C 32 min |
Vimentin | DAKO | V9 | 1:200 | Cook buffer CC1, 36 min at 95 °C | 37 °C 20 min |
Desmin | DAKO | D33 | 1:50 | Cook buffer CC1, 52 min at 95 °C | 37 °C 32 min |
Protein S100 | Zytomed | 4C4.9 | 1:3000 | Cook buffer CC1, 36 min at 95 °C | 37 °C 32 min |
CD34 | Immunotech | QBEND-10 | 1:500 | Cook buffer CC1, 52 min at 95 °C | 37 °C 48 min |
CK7 | DCS | OV-TL 12/30 | 1:1000 | Protease 1; 8 min | 37 °C 32 min |
CK20 | DAKO | Ks20.8 | 1:50 | Protease 1; 8 min | 37 °C 32 min |
E-Cadherin | BD Biosciences | Clone 36 | 1:2000 | Cook buffer CC1, 36 min at 95 °C | 37 °C 32 min |
β-Catenin | BD Biosciences | 14/β-Catenin | 1:50 | Cook buffer CC1, 64 min at 95 °C | 37 °C 32 min |
Ki67 | DAKO | MIB-1 | 1:100 | Cook buffer CC1, 52 min at 95 °C | 37 °C 32 min |
SMARCB1 | Zytomed | MRQ-27 | 1:50 | Cook buffer CC1, 36 min at 95 °C | 37 °C 60 min |
MLH1 | DAKO | ES05 | 1:50 | Cook buffer CC1, 64 min at 95 °C | RT, 100 min |
MSH2 | Ventana | G2-19-1129 | Prediluted | Cook buffer CC1, 64 min at 95 °C | 37 °C 60 min |
MSH6 | BD Pharmingen | MSH6 | 1:300 | Cook buffer CC1, 76 min at 95 °C | 37 °C 32 min |
PMS2 | DAKO | EP51 | 1:40 | Cook buffer CC1, 64 min at 95 °C | RT, 92 min |
