Figure 1

Reverse transcription (RT)-PCR testing for hepatitis E virus (HEV). (a) Scheme of semi-nested RT-PCR targeting the HEV open reading frame (ORF) 2/3 gene region. (b) Liver tissues available from patient 1: No amplification of HEV-specific sequences in the liver explant and day 1 biopsy, but in all following liver biopsies indicating chronic HEV infection acquired post-LT. (c) Direct sequencing of PCR products revealed HEV genotype 3 in all amplicons. Nucleotide sequences of the ORF 2/3 gene region varied among the four different patients, but were invariable comparing amplicons from different samples of the same patients.