Figure 4
The immune signature in alternative lengthening of macrophages and telomerase with macrophages was different. (a) Real-time quantitative PCR confirmed alternative lengthening of telomeres with macrophages (ALTM) tumors had increased expression of 3 interferon-induced protein with tetratricopeptide (IFIT) genes (1, 2, and 3) compared with telomerase with macrophages (TELM); alternative lengthening of telomeres (ALT), and telomerase (TEL) tumors. (b) Real-time quantitative PCR confirmed telomerase with macrophages tumors had increased expression of the scavenger receptor macrophage receptor with collagenous structure (MARCO), sushi-repeat containing protein, X-linked 2 (SRPX2), and CXCL12 compared with ALTM, TEL, and ALT tumors. Results mean, with the highest and lowest values included. ***P<0.001; ****P<0.0001. Tumor subgroups: ALT, an alternative lengthening of telomeres-associated promyelocytic leukemia body positive tumor with a low content of tumor-associated macrophages; ALTM, an alternative lengthening of telomeres-associated promyelocytic leukemia body positive tumor with a high content of tumor-associated macrophages; TEL a telomerase-positive tumor with a low content of tumor-associated macrophages; TELM, non-defined telomere maintenance mechanism tumor without alternative lengthening of telomeres-associated promyelocytic leukemia bodies that is potentially telomerase positive with a high content of tumor-associated macrophages.
