Figure 1 | Modern Pathology

Figure 1

From: Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization

Figure 1

Gastric cancers with co-amplification of ERBB2 and FGFR2 (Case 7), and ERBB2 and MYC (Case 9). In Case 7, amplification of ERBB2 and FGFR2 was found in different cancer cells. In Case 9, ERBB2 and MYC was in the same nuclei. Gene amplifications were analyzed by dual-color FISH (a: green fluorescence, ERBB2; orange fluorescence, FGFR2) (b: green fluorescence, ERBB2; orange fluorescence, MYC) and their protein overexpression was analyzed by immunohistochemistry (c and e, ERBB2; d, FGFR2; f, MYC). Panels a, c, and d show the same field. ERBB2- and FGFR2-amplified cells in panel a correspond to the 1+ ERBB2-stained cells in panel c and the cells with weak cytoplasmic staining of FGFR2 in panel d, respectively. The cells with gene amplification in panel b showed 2+ immunostaining of ERBB2 and equivocal nuclear staining of MYC.

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