Figure 2 | Modern Pathology

Figure 2

From: Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization

Figure 2

A poorly cohesive-type gastric cancer with FGFR2-amplified and MDM2-amplified cancer cells in different areas (Case 17). FGFR2 and MDM2 gene amplification was analyzed by dual-color FISH (a and b: orange fluorescence, FGFR2; green fluorescence, centromere 10. c: orange fluorescence, MDM2; green fluorescence, centromere 12) and their protein overexpression was analyzed by immunohistochemistry (d, FGFR2; E, MDM2). Panels a and b are different fields of the same area of panel d. Intense cytoplasmic staining of FGFR2 (d) was detected in both FGFR2-amplified (a) and FGFR2-non-amplified (b) cells. Panels c and e are the same area. Most but not all MDM2-amplified cells show 3+-nuclear staining of MDM2.

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