Figure 3 | Modern Pathology

Figure 3

From: Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization

Figure 3

Amplification and overexpression of MET in gastric carcinomas (Cases 21 and 22). A tubular adenocarcinoma (Case 21, a and b) and a poorly cohesive-type adenocarcinoma (Case 22, c–f) were analyzed for MET gene amplification by dual-color FISH (a, c, and d: orange signals, MET; green signals, centromere 7) and for MET protein overexpression by immunohistochemistry (b, e, and f). In the tubular adenocarcinoma (Case 21), cancer cells with 3+-immunostaining of MET displayed amplified MET; cells with negative immmunostaining displayed no amplification (a and b). The region enclosed by the black rectangle in panel b corresponds to the region shown in panel a. The poorly cohesive type adenocarcinoma (Case 22) consisted of MET-amplified (c) and non-amplified cancer cells (d) in different areas, and both areas displayed 2+-staining for MET by immunohistochemistry (e and f). Panels c and e, and panels d and f are the same field.

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