Table 2 Antibodies and dilutions applied for immunohistochemistry

From: Semi-comprehensive analysis of gene amplification in gastric cancers using multiplex ligation-dependent probe amplification and fluorescence in situ hybridization

Antibody

Clone

Dilution

Antigen retreval

Manufacturer

ERBB2

Polyclonal

1:400

None

Nichirei, Tokyo, Japan

EGFR

EGFR.25

1:100

PC pH6, citrate

Novocastra Lab, Newcastle, UK

FGFR2

ab58201

1:1000

None

Abcam, Cambridge, UK

MYC

Y69

1:50

PC pH9, EDTA

Pharmingen, San Diego, CA, USA

MDM2

IF2

1:40

PC pH6, citrate

Calbiochem, La Jolla, CA, USA

MET

SP44

1:50

PC pH6, citrate

Spring Bioscience, Pleeasanton, CA, USA

CCND1

SP4

Prediluted

PC pH6, citrate

Nichirei, Tokyo, Japan

  1. Abbreviation: PC, pressure cooking.
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