Figure 4
From: Distinct methylation profiles characterize fusion-positive and fusion-negative rhabdomyosarcoma
Pyrosequencing studies of six genes from the 11-gene signature. (a) Correlation between Infinium methylation score and pyrosequencing values. The methylation status of the specific CpG sites queried on the methylation arrays for six genes (MYOG, NHLH1, TGFB1, DIRAS3, LCP1, and EMILIN1) was analyzed by pyrosequencing in six fusion-positive and five fusion-negative samples, and compared with the β-value from the methylation array. (b) Distribution of methylation levels for multiple CpG sites in the six genes in fusion-positive and fusion-negative tumors. For each gene, methylation at the corresponding CpG site (filled circle) in the DNA methylation signature as well as its surrounding CpG sites (open circles) was measured by pyrosequencing in six fusion-positive and five fusion-negative samples. FN, fusion-negative rhabdomyosarcoma; FP, fusion-positive rhabdomyosarcoma.
