Table 1 Description of the FISH probes used to characterize MDM2, HMGA2, CDK4, and JUN amplification

From: Prognostic value of HMGA2, CDK4, and JUN amplification in well-differentiated and dedifferentiated liposarcomas

BAC clone a

Locus

Chromosomal location a

Position a

Origin

RP11-797C20

MDM2

12q15

69 161 511–69 340 249

CHORIb

RP11-30I11

Telomeric to 5′ region of HMGA2

 

65 892 238–66 063 441

CHORIb

RP11-299L9

5′ Region and exons 1 and 2 of HMGA2

 

66 049 805–66 225 867

CHORIb

RP11-23C9

5′ Region and exons 1, 2, and 3 of HMGA2

12q14.3

66 093 846–66 246 663

CHORIb

CTC-782I9

Intron 3 and exon 4 of HMGA2

 

66 235 316–66 355 707

Invitrogenc

CTD-2240K5

Exons 4 and 5 and 3′ region of HMGA2

 

66 325 027–66 457 979

Invitrogenc

RP11-118B13

3′ Region of HMGA2

 

66 358 701–66 502 988

CHORIb

RP11-571M6

CDK4

12q14.1

57 999 870–58 211 408

CHORIb

RP11-63G10

JUN

1p32.1

59 246 463–59 249 785

CHORIb

  1. ahttp://www.genome.ucsc.edu; UCSC Genome Browser on Human, February 2009.
  2. bCHORI: Children’s Hospital Oakland Research Institute (http://bacpac.chori.org/).
  3. cInvitrogen (Carlsbad, CA, USA).