Figure 1 | Modern Pathology

Figure 1

From: Influence of decalcification procedures on immunohistochemistry and molecular pathology in breast cancer

Figure 1

Overview of material and methods. Four tumor-containing biopsies were taken from breast tumor resection specimens (n=23). Each biopsy was placed in a different decalcifying agent, including EDTA, Christensen’s buffer with microwave (CBM), Christensen’s buffer without microwave (CB) and Formical-4 (F4). The original non-decalcified tumor was used as a control. Specimens were formalin fixed and paraffin embedded after which sections were cut for immunohistochemistry (IHC), fluorescence in situ hybridization (ISH) and DNA/RNA isolation.

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