Figure 4
Quality and quantity of RNA and DNA isolated from tissue pretreated with EDTA, Christensen’s buffer with (CBM) and without microwave (CB) and Formical-4 (F4; n=23). (a) 260/280 ratios for RNA, measured by Nanodrop. (b) Bioanalyzer RNA Integrity Number (RIN) values. (c) 260/280 ratios for DNA, measured by Nanodrop. (d) QFI (Quantitative Functional Index) in percentages (actual copy number/expected copy number, based on quantity of DNA measured by Nanodrop). (e) Average DNA fragment length in base pairs, measured with size ladder PCR. DNA input is corrected for amplifiable copy number, measured with the QuantideX qPCR assay. *P<0.05; **P<0.01; ***P<0.001.
