Figure 5

Yield of RNA and DNA, isolated from tissue from paired primary tumors and paired EDTA-treated bone metastases (n=8) measured with Nanodrop, Qubit and Bioanalyzer. Tumor areas were normalized and RNA and DNA yield corrected for tumor percentage. Paired differences were calculated with Wilcoxon signed-rank test. (a) RNA yield, measured by Nanodrop, Qubit and Bioanalyzer; (b) DNA yield measured by Nanodrop, Qubit and size ladder PCR.