Table 2 Comparison of ERα, PR (percentage of positively stained nuclei) and HER2 (DAKO scores) expression in paired samples of 23 breast cancers routinely processed (control) or undergoing decalcification in EDTA, Christensen’s buffer (CBM with or CB without microwave) or Formical-4 (F4)

Sample	ERα					PR					HER2
	Control	CBM	CB	EDTA	F4	Control	CBM	CB	EDTA	F4	Control	CBM	CB	EDTA	F4
1	100	100	100	100		30	1	5	5		1+	0	1+	0
2	90	100	100	100		1	0	0	0		0	0	1+	1+
3	90	100	100	100		1	0	0	0		0	0	0	0
4	100	90	100	100		100	100	75	90		2+	0	0	1+
5	100	90	75	100		90	75	90	100		3+	1+	2+	3+
6	0	0	0	0		0	0	0	0		3+	3+	3+	3+
7	90	50	75	90		100	90	100	10		0	0	0	0
8	97	90	90	100		97	100	100	90		0	0	0	0
9	100	100	100	100		0	0	0	0		0	0	0	0
10	100	100	90	100		65	75	65	35		0	0	1+	1+
11	0	0		5		0	0		0		0	0		0
12	100	75		90		60	20		75		0	0		0
13	100	95		75	75	90	95		65	65	0	0		0	0
14	100	100		100	75	100	100		100	75	0	0		0	0
15	100	75		75	75	100	100		100	90	0	0		0	0
16	35	75		35	50	0	75		65	75	3+	3+		3+	3+
17	100	75		90	90	5	75		90	65	0	0		0	0
18	1	0	10	0		10	10	2	10		0	0	0	0
19	90	75	100	20		100	90	100	75		0	0	0	0
20	100	90		90	90	50	35		50	50	1+	1+		1+	1+
21	100	100			100	65	90			90	0	0			0
22	100	100		95	95	65	65		90	90	0	0		0	0
23	100	95			75	100	95			75	0	0			0

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