Table 3 Linear weighted kappa of concordance between expression of ERα, PR and HER2 in split samples of 23 breast cancers routinely processed or undergoing decalcification in EDTA, Christensen’s buffer (with (CBM) or without microwave (CB)) or Formical-4

From: Influence of decalcification procedures on immunohistochemistry and molecular pathology in breast cancer

 

CBM

CB

EDTA

F4

ERα (1% cutoff)

0.646 (s.e. 0.324; 95% CI 0.012–1)

1

0.462 (s.e. 0.305; 95% CI 0–1)

1

ERα (10% cutoff)

1

0.625 (s.e. 0.333; 95% CI 0–1)

1

1

PR (1% cutoff)

0.701 (s.e. 0.193; 95% CI 0.324–1)

0.571 (s.e. 0.250; 95% CI 0.082–1)

0.696 (s.e. 0.195; 95% CI 0.314–1)

0

PR (10% cutoff)

0.679 (s.e. 0.170; 95% CI 0.346–1)

0.667 (s.e. 0.203; 95% CI 0.269–1)

0.667 (s.e. 0.176; 95% CI 0.323–1)

0

HER2 (neg vs pos)

0.777 (s.e. 0.213; 95% CI 0.360–1)

0.625 (s.e. 0.333; 95% CI 0–1)

1

1

HER2 (0, 1+, 2+, 3+)

0.782 (s.e. 0.126; 95% 0.536–1)

0.493 (s.e. 0.223; 95% CI 0.056–0.930)

0.800 (s.e. 0.106; 95% CI 0.593–1)

1

  1. (Almost) perfect concordance (0.8–1); substantial concordance (0.6–0.8); moderate concordance (0.4–0.6); poor concordance (0–0.4).
  2. Shown are the relative expression for ERα and PR with positivity according to 10 and 1% thresholds, the relative expression for HER2 with 3+ cases considered positive and the absolute expression for HER2 with exact DAKO scores (0, 1+, 2+ and 3+).
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