Figure 3
(a) Human phospho-kinase array analysis. Levels of phospho-kinases were assessed using a horseradish peroxidase-conjugated phospho-kinase antibody, which was followed by chemiluminescence detection. In T1-WT cells, the levels of phosphorylated Akt1/2/3, ERK1/2, and WNK1 were significantly reduced. Both T1-T602C and T1-G712A cells had significantly higher levels of phosphorylated Akt1/2/3 and WNK1. The level of phosphorylated ERK1/2 increased in T1-G712A cells and decreased in T1-T602C cells. *P<0.05 compared with cells expressing PPP2R1A-WT. (b) Evaluation of in vitro cell growth. For each experiment, 1.0 × 106 cells were cultured in RPMI and counted at 24, 72, and 120 h. *P<0.05 compared with cells expressing GFP; NS, not significant compared with cells expressing GFP.
