Table 4 Molecular features of pT1 colorectal carcinomas stratified by lymph node status and tumor budding
Molecular feature | Negative for lymph node metastasis (%) | Positive for lymph node metastasis (%) | P-valuea | Low tumor budding (0–4 per 0.95 mm2) (%) | High tumor budding (⩾5 per 0.95 mm2) (%) | P-valuea |
|---|---|---|---|---|---|---|
MMR protein and MSI PCR status | ||||||
Number of cases analyzed | 77 | 26 | 0.5 | 72 | 31 | 0.2 |
MMRP | 60 (78) | 22 (85) | 55 (76) | 27 (87) | ||
MMRD | 17 (22) | 4 (15) | 17 (24) | 4 (13) | ||
No. of cases analyzed by NGS | 26 | 22 | NA | 26 | 22 | NA |
Mean number of mutations (range) | 2.5 (0–5) | 2.6 (0–4) | 0.9 | 2.1 (0–5) | 3.0 (0-5) | 0.2 |
WNT pathway mutation | 16 (62) | 13 (59) | 0.9 | 16 (62) | 13 (59) | 0.9 |
APC | 16 (62) | 13 (59) | 16 (62) | 13 (59) | ||
CTNNB1 | 1 (4) | 0 | 1 (4) | 0 | ||
MAPK pathway mutation | 18 (69) | 14 (64) | 0.7 | 19 (73) | 11 (50) | 0.1 |
BRAF | 5 (19) | 3 (14) | 4 (15) | 4 (18) | 0.08 | |
KRAS | 10 (38) | 10 (45) | 13 (50) | 7 (32) | ||
NRAS | 3 (12) | 1 (5) | 3 (12) | 1 (5) | ||
Any RAS | 13 (50) | 11 (50) | 16 (62) | 8 (36) | ||
mTOR pathway mutation | 5 (19) | 4 (18) | 0.9 | 8 (31) | 1 (5) | 0.02 |
PIK3CA | 5 (19) | 3 (14) | 7 (27) | 1 (5) | 0.04 | |
AKT | 0 | 1(5) | 1 (4) | 0 | ||
PTEN | 0 | 0 | 0 | 0 | ||
TP53 mutation | 14 (54) | 13 (59) | 0.7 | 11 (42) | 16 (73) | 0.03 |
Other mutations | 4 (15) | 6 (23) | 0.3 | 4 (15) | 6 (27) | 0.3 |
CDKN2A | 1 (4) | 2 (9) | 1 (4) | 2 (9) | ||
SMAD4 | 0 | 1 (5) | 1 (4) | 0 | ||
FGFR3 | 1 (4) | 0 | 0 () | 1 (5) | ||
GNAS | 1 (4) | 1 (5) | 2 (8) | 0 | ||
RET | 0 | 1 (5) | 0 () | 1 (5) | ||
SMARCB1/c | 0 | 1 (5) | 0 () | 1 (5) | ||
FBXW7 | 0 | 1 (5) | 0 () | 1 (5) | ||
JAK3 | 1 (4) | 0 | 0 () | 1 (5) | ||
STK11 | 0 | 1 (5) | 0 () | 1 (5) | ||
