Figure 6

Interferon γ inhibits FABP1 expression in colorectal cancer cell lines. HT-29 and Colo205 cells were treated for 24 h with vehicle or IFNγ (10 ng/ml), or rosiglitazone (10 μM), or with the combination of both as indicated. (a) FABP1 mRNA expression is negatively correlated with interferon gamma response. A rank list of the Pearson correlated transcripts in the colorectal carcinoma data from The Cancer Genome Atlas was submitted to Gene Set Enrichment Analysis as a pre-rank file. The Interferon Gamma Response Hallmark was the top ranked negatively associated gene set. The FABP1 (b) and controls (c, d) mRNA expression level were determined by qRT–PCR upon treatment. The results are expressed as a fold change relative to vehicle treated cells. *Statistically significant P<0.05 and **statistically highly significant as P<0.001. Error bars represent s.d. Columns represent mean of three experiments. FABP1, fatty acid-binding protein 1; IFNγ, interferon gamma; qRT–PCR, quantitative real-time PCR.