Table 1 Immunohistochemical markers, clones, and protocols

From: Fatty acid-binding protein 1 is preferentially lost in microsatellite instable colorectal carcinomas and is immune modulated via the interferon γ pathway

Antibody

Vendor

Host

Antigen retrieval

Dilution

Detection method

PPARγ

Abcam

Cambridge, MA

Rabbit polyclonal

EDTA pH 9

Microwave

120 °C, 10 min

1:50

Dako Envision+Kit

CD3

Dako

Carpinteria, CA

Rabbit polyclonal

EDTA pH 9

Dako PTLink

97 °C, 20 min

RTU

Dako Envision FLEX Autostainer

FABP1

Abcam,

Cambridge, MA

Mouse monoclonal

Clone 2G4

EDTA pH 9

Dako PTLink

97 °C, 20 min

1:1000

Dako Envision FLEX Autostainer

  1. Abbreviations: FABP1, fatty acid-binding protein 1; PPARγ, proliferator-activated receptor γ; RTU, ready to use.
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