Figure 3

Comparison of different slide preparation and tumor-sampling techniques. Each point shows the difference between techniques plotted against the average of the two values. (a) Tissue microarray vs whole section from same tumor. Significant discrepancy was noted between tissue microarray and whole sections scores, with 95% limits of agreement lying at +17% and −44%. Endometrial cancer specimens were obtained from the same patient by blinded endometrial sampling performed immediately prior to surgery (pipelle) and after the uterus had been surgical removed (hysterectomy). (b) Whole slide scoring method. Significant variation between the two tumor-sampling techniques was noted using whole slide scoring (95% limits of agreement −18 to +38%). (c) Hot spot scoring method. In contrast, hot spot scoring appeared more consistent, with the exception of a single outlier.