Table 1 Comparison of manual and semi-automated scoring of Ki-67 expression

From: Ki-67 in endometrial cancer: scoring optimization and prognostic relevance for window studies

Criteria

Manual scoring n=33

Semi-automated scoring n=179

  

Whole slide scoring

Hot spot scoring

Invasive edge scoring

Intra-observer intra-class correlation coefficient

0.91

0.96

0.96

0.75

Inter-observer intra-class correlation coefficient

0.92

0.92

0.91

0.87

Percentage of slides possible to score in cohort

100

100

100

50

Time per slide (min)

7.7

3.1 (+2 min to run solution)

2.3 (+2 min to run solution)

2.2 (+2 min to run solution)

  1. Invasive edge scoring had lower intra- and inter-observer reproducibility than manual, whole slide or hot spot scoring and could only be performed on the 50% of available slides in which the endometrial–myometrial interface was sampled. Manual scoring had ‘almost perfect’ intra- and inter-observer reproducibility but took considerably longer to perform than whole slide and hot spot scoring using the Definiens Developer algorithm.
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