Figure 1
From: microRNA in situ hybridization for miR-211 detection as an ancillary test in melanoma diagnosis
Intradermal nevus nests (arrows) and overlying epidermal keratinocytes diffusely and strongly express miR-211 (cytoplasm, red; and nuclei, blue) detected by fluorescent miRNA in situ hybridization (a). In contrast, the pagetoid, epidermal melanoma cells (circles and arrows) are negative for miR-211 (cytoplasm, black; and nuclei, blue) in a melanoma in situ (b). Measured fluorescent intensity showed a significant decrease in miR-211 expression in primary cutaneous melanomas compared with common nevi (P-value<0.0001) (c). The fluorescent intensity was expressed in boxplots: box starts from first quartile and ends at third quartile; cross represents mean; line represents median; the ends of whiskers represent the minimum and maximum of the data that are not outliers; and black dots are outliers. Receiver operating characteristic curve showed an accurate discrimination between diagnostic primary cutaneous melanoma and common nevi groups (area under the curve=0.853) (d). Original magnifications: (a–b), × 200.
