Figure 2
From: microRNA in situ hybridization for miR-211 detection as an ancillary test in melanoma diagnosis
Coalescing intradermal nevus nests diffusely expressed miR-211 (cytoplasm, red; and nuclei, blue) (a) compared with melanoma in situ (arrowheads) and invasive dermal (circles) tumor cells are negative for miR-211 (cytoplasm, black; and nuclei, blue) (b). The insets show negative control (miRNA scramble), devoid of any background or non-specific signal (cytoplasm, black; and nuclei, blue). Serial sections (hematoxylin and eosin) are shown for the two lesions in panels (a) and (b), respectively (c and d). Melanin did not interfere with the miR-211 hybridization or signal detection (d). Dysplastic nevi diffusely expressed miR-211 both in the junctional and dermal components (e). Serial section is shown (case no. 20) from the scalp of a 26-year-old woman (f); the inset shows high magnification of the junctional melanocytic atypia. Original magnifications: (a–d), × 200; and (e) and (f), × 100.
