Figure 3
From: A FISH assay efficiently screens for BRAF gene rearrangements in pancreatic acinar-type neoplasms
Representative immunohistochemical staining for pERK and corresponding morphology feature and BRAF rearrangement FISH analysis finding. (a) A pure acinar cell carcinoma case with solid growth pattern, expressing nuclear and cytoplasmic pERK labeling. BRAF rearrangement was detected by the FISH assay showing multiple ‘single orange (3′BRAF)’ signals; (b) a mixed acinar ductal carcinoma case, positive for pERK staining. BRAF rearrangement was detected by the FISH assay showing ‘broken apart’ signal pattern; (c) a pure acinar cell carcinoma case with glandular growth pattern is focally positive for pERK by immunohistochemistry. BRAF rearrangement was not detected by the FISH assay, but the FISH assay revealed BRAF gain/amplification.
