Figure 2

Staining of macrophages and lymphocytes. Original magnifications × 200. (a,b) A case with macrophages negative for PD-L1. (c,d) A case with macrophages with cytoplasmic PD-L1 staining. (e,f) A case with macrophages with membranous PD-L1 staining (the most common finding). (a,c,e) Stained with hematoxylin–eosin. (b,d,f) Stained with 22C3 and 28-8, respectively. (g,h) Lymphocytes positive for PD-L1, SP142, and 22C3, respectively.