Figure 2
Molecular alteration in peritumoral vs normal tissue of head and neck squamous cell carcinoma patients. (a) H&E staining performed on formalin-fixed paraffin-embedded tissue slices showing the histological analysis of peritumoral and tumoral areas from three representative head and neck squamous cell carcinoma patients. (b) Supervised clustering analysis representing the genes differentially expressed between 22 tumor tissues vs their normal counterparts (P<0.05). Expression of these genes in peritumoral tissues is also shown. (c) Supervised clustering analysis representing the miRNAs differentially expressed between 66 tumor tissues vs their normal counterparts (P<0.05, fold change >2). The expression matrix shows the behavior of these miRNAs also in peritumoral tissues. (d) Box plot showing the overall expression of the miRNAs differentially expressed between 66 peritumoral tissues and their normal counterparts (P<0.05). (e) Box plots showing the expression of four selected miRNAs (miR-429, miR96-5p, miR-21-5p, and miR-21-3p) evaluated by RT-qPCR in 132 peritumors vs 18 normal samples from head and neck squamous cell carcinoma patients.
